The durability test system for vacuum booster with master cylinder based on USB2828 data acquisition card was introduced. The specific method of changing output pressure endurance testing was detailed. The test system was completely different from the test methods prescribed by the automotive industry standards and had a certain degree of innovation. The practical results show that the operation is stable and reliable, the input force is controlled accurately, and the hydraulic and vacuum data acquisition is performed in real time. It can meet the requirements for the durability test of the vacuum booster with master cylinder by the car manufacturers.