Hydrostatic guide as a key component of the precision machine tool, its performance parameters directly affects the overall performance of machine tool. Using singlepoint excitation, multipoint pickup method, the space frequency response of the hydrostatic guide on the three directions was obtained. And using an improved frequency response function method, the identification algorithm was compiled, the dynamic characteristics parameters of the hydrostatic guide were identified. The modal mass matrix, stiffness matrix, viscous damping matrix, structure damping matrix of the three directions in hydrostatic guide junction space were identified.It is found on the rail slide vertical Z direction, the orders of magnitude of the stiffness matrix, viscous damping matrix increase nonlinearly with excitation frequency increasing, dynamic parameters on rail feed X and side Y directions less affect by high frequency excitation frequency.