In the case of high speed rotating spindle, a multiDOF hydraulic load experimental station was designed to measure the spindle rotation accuracy and the dynamic stiffness under load conditions, applying the theory that hydraulic cylinder had a large thrust. Since the experimental station had multiDOF, it could be used to multidirectional load,its hydraulic pump and relief valve also could be used to change the loading force easily. The experimental station can be widely used in performance test of lathes and other machine tools.