The electrohydraulic fatigue testing machine is widely applied in low frequency fatigue testing in the engineering field due to its large output load. However, its testing frequency can not be furthered increased because of the frequencywidth limitation of the conventional electrohydraulic servovalve. The two dimensional (2D) high frequency rotational valve characterized by its rapid frequency response and high flow rate, is a 2DOF rotational valve, whose valve spool is able to achieve both axis linear motion and rotation. The fatigue testing project of asymmetrical hydraulic cylinder controlled by 2D high frequency rotational valve was proposed. The fatigue testing frequency could be increased effectivly of the vertical electrohydraulic fatigue testing machine. The resonant fatigue testing solution was proposed in order to solve the amplitude decay problem of the load of high frequency. Based on the mode theory, the displacement equation was analyzed of the upward and downward grips of the electrohydraulic fatigue testing machine during resonance, and simulation models were made. Research results show the feasibility of resonant fatigue testing through solution of asymmetrical hydraulic cylinder controlled by 2D high frequency rotational valve, which provides guidance for future experimental studies.