Taking the industrial robot sorting workstation as the research object, aiming at the difficulty of its trajectory planning and automatic production coordination,the application of ABB Robot Simulation software RobotStudio in the industrial robot automatic sorting workstation is proposed. The Three Dimensional (3D) entity model and layout were built. The virtual controller technology of ABB was used to import it into RobotStudio, and the 3D entity model and overall layout of the automatic sorting workstation were built. Smart component of hand claw cylinder was added of sub-components and set of attributes to create robot I/O signal and connection mode, to simulate different gesture actions of cylinder through time manager, and to set logical relationship between robot and hand claw cylinder. Off-line programming and Simulation of machine manual workstation were completed. It can provide technical reference and feasibility basis for the design and manufacture of robot sorting workstation, reduce the cost of production line design and debugging, and guide the field production.