Aiming at the phenomenon that the measurement efficiency and accuracy of the radial, roll angular and pitch angular stiffness of rolling linear guide pair were hard to balance,a measurement method with high efficiency and high accuracy was proposed, by which radial stiffness, roll angular stiffness and pitch angular stiffness of the guide rail could be accurately measured through one operation and the device was designed.Using this method, first the disturbance term was isolated and the measurement of the radial and roll angular static stiffness was separated from the pitch angular static stiffness, and then the loading force and the deformation of the guide rail pair were decomposed into the vertical, inclined and pitching directions by using the parameter separation technique. Finally the highefficiency and highprecision measurement of the three static stiffness was realized.The results show that by using the device and method, the efficiency and accuracy of the static stiffness measurement of the guide rail can be effectively improved.Compared with the existing mature technology, the efficiency is raised by 81%. The accuracy of radial, roll angular and pitch angular stiffness is raised by 17%, 17.6%, 18.9% respectively. It provides reference for improving the efficiency of static stiffness evaluation of rolling linear guide pair and the development of high rigidity design.