Multi-opposed plunger pump is a new type of product with the advantages of high pressure, large flow, high efficiency and energy saving, but because of its novel and complex structure, existing methods can not be used to balance its disturbance. Aiming at the special structure of multi-opposed piston pump, the main moving mechanism of multi-opposed plunger pump was analyzed and the calculation formula of disturbance force was deduced. Then different balancing methods were designed according to the characteristics of disturbance force to balance the disturbance force. An effective method for balancing the disturbance force of multi-opposed plunger pump was put forward. The simulation results show that the balancing method has practical value for the disturbance balance of multi-opposed plunger pumps.