Aimed at the problem that the control system of permanent magnet DC motor for electric bus was subject to motor parameter perturbation, gear change and load change, the regenerative braking of electric bus in driving and sliding condition was studied on the basis of establishing the main loop of control system,the robust H∞ control scheme based on genetic algorithm was proposed. The minimum interference and good robustness of the control system were taken as control objectives, the selection of the weighted function in the robust H∞ controller was transformed into a multi-objective optimization problem. The optimal robust H∞ controller was designed by using the genetic algorithm to optimize.The experimental results show that the bus with robust H∞ controller has a faster speed response during driving than the bus with double closed loop PI control, and has better stability, stronger robustness and anti-interference ability than the bus with double closedloop PI control during regenerative braking in downhill sliding condition