The accuracy of RV reducer transmission is mainly affected by the transmission accuracy. Taking the high precision RV80E reducer as the research object, aiming at its structural characteristics, the rigidflexible coupling model of RV reducer is established by using ADAMS multibody dynamics simulation software, and the correctness of the model is verified. Meanwhile, the rigidflexible coupling model is used to design several groups of simulation prototypes with different errors, and the influence of different error factors on the transmission accuracy of the whole machine is analyzed by simulation results. Through different error factors, it is concluded that the error combination of positive equidistance+negative offset or negative equidistance+positive offset can effectively reduce the influence of transmission error on the transmission accuracy of the whole machine, Finally, the main factors that affect the accuracy of the second stage transmission are obtained through the experimental test, which provides a certain theoretical basis for the production and manufacture of RV Reducer.