Aiming at the positioning accuracy of 6-DOF robot,a kinematic parameter calibration method was proposed based on the robot kinematic error model.The pre-identification of robot kinematics parameter deviation was realized by using Levenberg-Marquardt algorithm.The suboptimal value of these identified parameter deviations was defined as the central value of the initial individual component,and the differential evolution algorithm was used to complete the accurate identification,so as to obtain the better value of robot kinematics parameters.In order to verify the effectiveness of the proposed method,the experimental study of motion parameter calibration was carried out by using laser tracker and numerical simulation.The experimental results show that the proposed kinematic parameter calibration method can be used to significantly improve the positioning accuracy of 6-DOF robot.