Under the condition of cutting fluid, single factor test method was used to mill GH4169 with coated carbide end mill, and the influence of cutting parameters on milling vibration was analyzed. The experimental results show that the influence of single factor method on milling vibration is different in the range of the experimental conditions, and the milling vibration values from large to small are vx, vy and vz respectively;the influence of cutting speed vc and feed rate fz is large; the influence of radial cutting depth ae is not obvious; the axial cutting depth ap has great influence on milling vibration.