Aimed at the problem of tension fluctuation of reciprocating mono-wire saw in running, the influence of leading wire spool's rotational acceleration on wire tension was proposed and analyzed. The mathematical model of wire winding system was built, and the relationship between spool's rotational acceleration and tension fluctuation were revealed. A dynamic simulation model was built with the Matlab/Simulink, and three kinds of reversing method were used to explore the tension fluctuation through simulation. The simulation results show that the influence rule of tension fluctuation and spool's rotational acceleration is direct. The double cosine half-wave reversing method has the minimum tension fluctuation by compared with three kinds of simulation results.