In accordance with the production characteristics of the existing automobile assembly line, the RFID middleware system application requirements in the assembly line were analyzed, a set of RFID middleware application system architecture oriented to auto assembly line was established, the functions and typical structure of the RFID middleware were described. Several keys, such as the assembly line system, management to read and write device, data filtering, message transmission etc, were descripted. The whole RFID middleware platform was developed using JAVA technology. 