As a kind of common polymeric materials, polydimethylsiloxane (PDMS) has become widely used in microfluidic chips. In this paper, a set of fabrication process of the microfluidic chips is presented which doesn’t need clean-rooms, by comparing with the conventional microfluidic processing technology. It includes the fabrication of PDMS microchannel structure, the treatment of surface wettability, and the multilayer bonding technology and its application. This economical processing technology is suitable for the most common laboratory.