Tissue engineering scaffold's structure, spatial trend, porosity is an important factor that restricts the development of tissue engineering. The microfluid flow field in the scaffold has a direct influence on the adsorption, proliferation, differentiation of cells and the stress and strain induced by fluid-structure coupling. Because of the complex interaction of various factors in the field, the functional mechanism is not yet clear. The research on the micro-fluid flow characteristics in the inner micro-pore is still in initial stage. By elaborated the research situation of the internal flow field in the bone tissue engineering scaffold and the interaction between fluid shear stress and neo-tissue, it was found that at the same time of the numerical method used to simulate the phenomenon, it was also lack of corresponding physical experiment. The development of Rapid prototyping technology and new materials has a certain engineering significance to build a micro-fluid experimental platform, to explore the optimization of bone scaffold and internal micro-fluid flow field.